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派博传思国际中心 »期刊书目

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Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe

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发表于 2025-3-21 18:49:55 | 显示全部楼层 |阅读模式
书目名称Homing Endonucleases
副标题Methods and Protocol
编辑David R. Edgell
视频videohttp://file.papertrans.cn/429/428071/428071.mp4
概述Serves as a comprehensive resource to identify and characterize homing endonucleases and much more.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation
丛书名称Methods in Molecular Biology
图书封面Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe
描述.Homing Endonucleases: Methods and Protocols. aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Homing Endonucleases: Methods and Protocols. serves as a key reference for all labs studying site-specific DNA endonucleases..
出版日期Book 2014
关键词Binding and cleavage specificity; Genome editing; Mobile genetic elements; Recombination-based pathways
版次1
doihttps://doi.org/10.1007/978-1-62703-968-0
isbn_softcover978-1-4939-6319-5
isbn_ebook978-1-62703-968-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleasefore, it can be used for assaying in vivo activity of homing endonucleases. Moreover, due to its high sensitivity, it can be applied for directed evolution of homing endonucleases with altered sequence specificity.
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Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile,ingle experiment. The base preference at each position of the target sequence can be quantitated based on this assay, and the overall binding specificity of the enzyme can thereby be determined and compared with its cleavage specificity.
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Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers, homing endonucleases and discuss their strengths and weaknesses in genome editing as compared to other site-specific nucleases such as zinc finger endonucleases, TALEN, and CRISPR-derived endonucleases.
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Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display, assumptions or knowledge regarding the target site. This protocol is easily integrated into the yeast surface display pipeline and is leveraged by the expansive number of existing tools for both SELEX and yeast surface display.
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Bioinformatics Identification of Coevolving Residues,e sequence alignments for coevolution inference. Furthermore, it is important to understand common signs and sources of error. When confounding factors are accounted for, coevolution is a rich resource for protein engineering information.
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