Titlebook: High-Throughput Screening Assays in Toxicology; Hao Zhu,Menghang Xia Book 2016 Springer Science+Business Media New York 2016 Chemical toxi

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Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor ActivityXR signaling can be used as therapeutic candidates or identified as potentially hazardous compounds depending on exposure doses and health states. Therefore, there is an increasing need for high-throughput screening assays of FXR activity to profile large numbers of environmental chemicals and drugs

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Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cellsn hepatoma HuH-7 cells develop into a differentiated in vitro model resembling primary human hepatocytes after a 2-week dimethyl sulfoxide (DMSO) treatment. DMSO-treated HuH-7 cells express elevated cytochrome P450 3A4 (CYP3A4) enzyme gene expression and activity compared to untreated HuH-7 cells. T

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Determination of Histone H2AX Phosphorylation in DT40 Cellsd blocked replication forks induce the phosphorylation of H2AX at serine 139 (γH2AX), and accumulate γH2AX which can then be detected as foci. The detection of γH2AX foci by immunostaining with antibodies that recognize γH2AX is an indicator of DSBs presence. This chapter describes the measurement o

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Fast Functional Germline and Epigenetic Assays in the Nematode ,y of their genome are therefore of utmost importance for the health of organisms and survival of species. Many features of mammalian germ cells, including their long development span and difficulty of access, present challenges for their study in the context of toxicity assays. In light of these bar

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Correction of Microplate Data from High-Throughput Screeningand cost-effective manner. The errors and noises in the microplate-formatted data from HTS have unique characteristics, and they can be generally grouped into three categories: run-wise (temporal, multiple plates), plate-wise (background pattern, single plate), and well-wise (single well). In this c

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CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprintsta normalization, additional parsing is often needed to account for incomplete read outs or various artifacts that arise from signal interferences..CurveP is a heuristic, user-tunable, curve-cleaning algorithm that attempts to find a minimum set of corrections, which would give a monotonic dose–resp