| 书目名称 | High-Throughput RNAi Screening | | 副标题 | Methods and Protocol | | 编辑 | David O. Azorsa,Shilpi Arora | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In .High-Throughput RNAi Screening: Methods and Protocols,. expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfal | | 出版日期 | Book 2016 | | 关键词 | drug discovery; short interfering RNA (siRNA); short hairpin RNA (shRNA); genomics; HT-RNAi; mammalian ce | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6337-9 | | isbn_softcover | 978-1-4939-8168-7 | | isbn_ebook | 978-1-4939-6337-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2016 |
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发表于 2025-3-21 17:05:49
