| 书目名称 | High-Throughput Glycomics and Glycoproteomics | | 副标题 | Methods and Protocol | | 编辑 | Gordan Lauc,Manfred Wuhrer | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, .N-.glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest .N.-glycans, as well as plant glycans. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introduction to their respective topics, | | 出版日期 | Book 2017 | | 关键词 | glycosylation; glycans in eukaryotic life; tandem mass spectrometric analysis of protein glycosylation | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6493-2 | | isbn_softcover | 978-1-4939-8217-2 | | isbn_ebook | 978-1-4939-6493-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2017 |
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发表于 2025-3-21 18:39:38
