| 书目名称 | High-Resolution Profiling of Protein-RNA Interactions |
| 编辑 | Mathias Munschauer |
| 视频video | http://file.papertrans.cn/427/426731/426731.mp4 |
| 概述 | Nominated as outstanding PhD thesis by Max-Delbrück Center for Molecular Medicine, Germany.Describes the development of a novel technology for transcriptome-wide analysis of protein-RNA interactions.P |
| 丛书名称 | Springer Theses |
| 图书封面 |  |
| 描述 | .The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative .cis.-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several jour |
| 出版日期 | Book 2015 |
| 关键词 | LIN28B Binding Sites; Next-generation Sequencing; PAR-CLIP; Post-Transcriptional Regulatome; Post-transc |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-319-16253-9 |
| isbn_softcover | 978-3-319-36848-1 |
| isbn_ebook | 978-3-319-16253-9Series ISSN 2190-5053 Series E-ISSN 2190-5061 |
| issn_series | 2190-5053 |
| copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl |
|Archiver|手机版|小黑屋|
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