书目名称 | High-Resolution Imaging of Cellular Proteins |
副标题 | Methods and Protocol |
编辑 | Steven D. Schwartzbach,Omar Skalli,Thomas Schikors |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful .Methods in Molecular Biology series. format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.. |
出版日期 | Book 2016 |
关键词 | molecular tool box; epitope tagged proteins; polyclonal antibodies; anti-peptide antibodies; fluorescent |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6352-2 |
isbn_softcover | 978-1-4939-8173-1 |
isbn_ebook | 978-1-4939-6352-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2016 |