Titlebook: High Throughput Screening; Methods and Protocol William P. Janzen Book 20021st edition Springer Science+Business Media New York 2002

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,Homogeneous Techniques for Monitoring Receptor–Ligand Interactions,-surface receptors (.). The initial studies relied on laborious, vacuum-filtration techniques that are cumbersome and difficult to automate. Despite these limitations, filtration-based radioligand binding assays were adequate to probe the kinetics of receptor-ligand interactions and to screen small

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Comparison of SPA, FRET, and FP for Kinase Assays,lecule libraries and natural products (.–.). Many of high throughput kinase assays have been developed in 96-, 384-, and 1536-well formats using these technologies (.–.). Development of these technologies allow one to quickly large synthetic compound or natural product libraries in a very short peri

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Cellular Assays in HTS,y in the 1940s, 1950s, and 1960s (.). However, cellular assays also represent one of the most dynamic areas of innovation in high-throughput screening (HTS). The driver of this innovation is the demand for more leads from more complex targets. Genomics discovery efforts have generated many potential

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Compound Library Management,d screening technologies are a response to the need of more and better quality compounds in the industry pipelines. But these inmense collections (from several hundred thousands to several millions of compounds) pose a tremendous logistical problem to be overcome in order to harvest all their tre-me

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Natural Products or Not? How to Screen for Natural Products in the Emerging HTS Paradigm, in the world are natural products or their derivatives (.). Roughly 60% of the antitumor and anti-infective agents that are available commercially or were in latestage clinical development from 1989–1995 are of natural products origin (.,.).

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Introduction to Screening Automation, 1) small scale servo-driven robotic devices; 2) the personal computer; and 3) the microplate. The word “robot” is derived from the Czechoslovakian word Robota, which is translated into English as servant, slave, or laborer. The Robot Institute of America (RIA) defines a robot as a “reprogrammable m

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Unit Automation in High Throughput Screening,omation is best implemented only to the extent that operations are standardized, i.e., if you standardize individual assay steps (or unit operations) they can be readily (and effectively) automated (.). If entire assay formats could be standardized, then it would make sense to automate the entire as

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Management and Maintenance of High Throughput Screening Systems,apidly and in the most cost effective manner possible. The most reliable way this can be achieved is by rigorous attention to the quality of the assays themselves, the quality of the compounds used, as well as the quality and condition of the equipment used. To effectively reach this goal all of the

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1064-3745 diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wish had been available when they started in HTS, these expert seasoned authors illuminate the HTS process with richly detailed tutoria