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Titlebook: High Throughput Protein Expression and Purification; Methods and Protocol Sharon A. Doyle Book 2009 Humana Press 2009 Cloning.Escherichia c

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书目名称High Throughput Protein Expression and Purification
副标题Methods and Protocol
编辑Sharon A. Doyle
视频video
概述Provides step-by-step protocols for protein production that utilize a wide range of cutting-edge techniques.Covers cell-based and cell-free methodologies needed to produce bacterial proteins, eukaryot
丛书名称Methods in Molecular Biology
图书封面Titlebook: High Throughput Protein Expression and Purification; Methods and Protocol Sharon A. Doyle Book 2009 Humana Press 2009 Cloning.Escherichia c
描述.Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In .High Throughput Protein Expression and Purification: Methods and Protocols., leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. ..Cutting-edge and comprehensive, .High Throughput Protein Expression and Purification: Methods and Protocols. is an i
出版日期Book 2009
关键词Cloning; Escherichia coli; Genome sequencing; PCR; Parallel processing; membrane proteins; molecular biolo
版次1
doihttps://doi.org/10.1007/978-1-59745-196-3
isbn_softcover978-1-61737-821-8
isbn_ebook978-1-59745-196-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
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