书目名称 | Heterologous Protein Production in CHO Cells |
副标题 | Methods and Protocol |
编辑 | Paula Meleady |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Comprehensive and cutting-edge, .Heterologous Protein Production in CHO Cells: Methods and Protocols .is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.. |
出版日期 | Book 2017 |
关键词 | CRISPR; Cas9; bioinformatic; recombinant protein; CHO-K1 |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6972-2 |
isbn_softcover | 978-1-4939-8356-8 |
isbn_ebook | 978-1-4939-6972-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media LLC 2017 |