| 书目名称 | Heterologous Gene Expression in E.coli | | 副标题 | Methods and Protocol | | 编辑 | Thomas C. Evans, Jr.,Ming-Qun Xu | | 视频video | | | 概述 | Helps to guide scientists toward an ideal expression host.Provides easy to follow protocols, ready to be implemented in the lab.Includes tips from the experienced, expert contributors on troubleshooti | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry.Despite the advanced state of protein expression technology improvements are still needed.For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host.The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli.In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host.The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins.Written in the highly successful Methods in Molecular Biology™ format, chapters include intro | | 出版日期 | Book 2011 | | 关键词 | Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-61737-967-3 | | isbn_softcover | 978-1-4939-5725-5 | | isbn_ebook | 978-1-61737-967-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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发表于 2025-3-21 19:23:13
