| 书目名称 | Heterologous Gene Expression in E.coli | | 副标题 | Methods and Protocol | | 编辑 | Nicola A. Burgess-Brown | | 视频video | | | 概述 | Includes cutting-edge methods and protocols for the study of E. coli gene expression.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Heterologous Gene Expression in E. coli: Methods and Protocols .serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.. | | 出版日期 | Book 2017 | | 关键词 | Protein purification; Recombinant proteins; Escherichia coli; Structural biology; High-throughput clonin | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6887-9 | | isbn_softcover | 978-1-4939-8328-5 | | isbn_ebook | 978-1-4939-6887-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media LLC 2017 |
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发表于 2025-3-21 18:51:52
