| 书目名称 | Heterologous Expression of Membrane Proteins | | 副标题 | Methods and Protocol | | 编辑 | Isabelle Mus-Veteau | | 视频video | | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using .E. coli. as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition. serves as an ideal guide f | | 出版日期 | Book 2022Latest edition | | 关键词 | Bacterial host models; Protein expression; Production systems; Structural studies; Protein purification | | 版次 | 3 | | doi | https://doi.org/10.1007/978-1-0716-2368-8 | | isbn_softcover | 978-1-0716-2370-1 | | isbn_ebook | 978-1-0716-2368-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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发表于 2025-3-21 19:37:35
