Titlebook: Herpes Simplex Virus; Methods and Protocol Russell J. Diefenbach,Cornel Fraefel Book 2020Latest edition Springer Science+Business Media, LL

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Herpes Simplex Virus Growth, Preparation, and Assay,emely contagious viruses with a high seroprevalence in the human population emphasizing the importance of studying their biology. Hence, the propagation and purification of virus stocks constitute a key element in laboratory work.

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Engineering HSV-1 Vectors for Gene Therapy,bera (Alipogene tiparvovec) as the first gene therapy product as a standard medical treatment (Yla-Herttuala, Mol Ther 20:1831–1832, 2013), gene therapy has reached the status of being a part of standard patient care. Replication-competent herpes simplex virus (HSV) vectors that replicate specifical

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Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors,gene transfer tools stems from the fact that the genomes of these vectors do not carry protein-encoding viral sequences. Consequently, they are completely safe for the host and nontoxic for the infected cells. Moreover, the complete absence of virus genes provides a genomic space authorizing a very

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HSV-1 Amplicon Vectors as Genetic Vaccines,vectors encoding structural proteins from both foot-and-mouth disease virus and rotavirus were partially protected from challenge with wild-type virus (D’Antuono et al., Vaccine 28:7363–7372, 2010; Laimbacher et al., Mol Ther 20:1810–1820, 2012; Meier et al., Int J Mol Sci 18:431, 2017), indicating

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Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein,nerate, the replicating recombinant virus, and the methods to purify it and characterize it for the correct expression of the transgene. We present the example of R-115, a recombinant expressing murine interleukin 12 (mIL12) from the US1–US2 intergenic region. A specific method for the production of

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Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISation potential discrepancies in their regulation according to the nuclear positioning. Latent herpes simplex virus 1 (HSV-1) genome remains as an episome in the nucleus of the infected neurons. Accordingly, depending on the location of the viral genomes in the nucleus, they could be targeted by dif