Titlebook: Herpes Simplex Virus; Methods and Protocol Russell J. Diefenbach,Cornel Fraefel Book 2014 Springer Science+Business Media New York 2014 Can

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Andrea S. Laimbacher,Cornel Fraefelfictional voices that integrate complex theoretical and analytical traditions in curriculum studies and pedagogy. This chapter also references teaching work done with high school students at a zoo several years ago where the author employed fiction as a way to explore the meaning of the captive animal in the zoo.

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Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components,s and β-sheets of individual subunits can be recognized. A relaxation of the symmetry in the reconstruction steps allows us to resolve the DNA packaging protein located at one of the 12 vertices in the capsid.

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Book 2014asic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport an

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Isolation of Herpes Simplex Virus Nucleocapsid DNA,tenated forms and may have modifications such as histones that are added within cells. During this protocol, the viral capsid protects the HSV genome from reagents that strip away and destroy most cellular contaminants. This procedure describes the isolation of viral nucleocapsids and their subsequent dissolution to purify clean, linear HSV DNA.

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Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals,mplification and sequencing. This approach has the advantage of being faster, but only frameshift mutations and stops of translation can be interpreted without doubt and numerous amino acid substitutions are diagnostically less conclusive.