Titlebook: Hepatocytes; Methods and Protocol Naoki Tanimizu Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Generation of Hepatocyte Organoids from Human iPS Cells, for a number of degenerative diseases. In this chapter, I describe a strategy to mass-produce fully functional hepatocyte organoids using hiPSCs interlaced with human adipose microvascular endothelial cells (HAMEC). Our unique technology employs a two-step strategy, consisting of the scalable gener

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Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver Progenitor Cells and Livearious liver cells. We have already developed culture systems to generate liver progenitor cells (LPCs), hepatocytes, and liver sinusoidal endothelial cells (LSECs) from human induced pluripotent cells (iPSCs). Using those cells, we established a co-culture system to study HBV infection and replicat

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In Vitro Assay System to Detect Drug-Induced Bile Acid-Dependent Cytotoxicity Using Hepatocytes,n vitro assay system to detect bile acid-dependent cytotoxicity in hepatocytes. This cell-based system can assess the toxicity of the parent compound, as well as the contribution of metabolite(s). In addition, this system can utilize several types of hepatocytes (primary hepatocytes, hepatoma cell l

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Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes,on recombinant adenovirus-mediated infection combined with a pulse-chase regimen and live-cell imaging analysis of fluorescent protein-tagged dipeptidyl peptidase IV (DPPIV) and vesicular stomatitis virus G (VSVG) cargo proteins, coexpressed and accumulated in the endoplasmic reticulum via DPPIV agg

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Assessment of Hepatocyte Ploidy by Flow Cytometry,in the distribution of diploid and polyploid cells in the liver can be indicative of tissue stress or a pathologic state. Here, we describe a method for flow cytometric quantification of ploidy distribution by staining with propidium iodide. We first outline a hepatocyte isolation procedure from mou

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Isolation of Small Hepatocyte-Like Progenitor Cells from Retrorsine/Partial Hepatectomy Rat Livers ers treated with retrorsine (Ret) and a 70% partial hepatectomy (PH). The Ret/PH model has been used widely to analyze the effectiveness of cell transplantation and the mechanisms of LSPC proliferation. Laser microdissection (LMD) is a powerful tool that can excise and collect specific areas of cell