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Titlebook: Hepatocytes; Methods and Protocol Naoki Tanimizu Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Hepatocytes978-1-0716-2557-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations,Collagen sandwich culture of hepatocytes retains many in vivo-like properties and is used for many investigations in liver cell biology, and hepatic pharmacology and toxicology. This chapter describes the method of establishing collagen sandwich culture of hepatocytes in a glass bottom dish for image-based studies.
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Feng Chene implied assumption that the parties have not by agreement extended the scope of the Convention. But, as has already been pointed out,. it is not uncommon that the owner and the charterer agree that the Warsaw Rules shall apply to a contract which would not automatically have been governed by them.
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Yuji Suzuki,Tokio Sasaki,Keisuke Kakisaka,Hiroaki Abe,Yasuhiro Takikawa done by discussing some practical situations in order to find out where the Warsaw Convention, according to our interpretation, applies. The intention is by no means to cover all the possible combinations of circumstances and arrange the material systematically, but only to pick out some situations that seem practical or otherwise interesting.
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Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells, This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.
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Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver Progenitor Cells and Livearious liver cells. We have already developed culture systems to generate liver progenitor cells (LPCs), hepatocytes, and liver sinusoidal endothelial cells (LSECs) from human induced pluripotent cells (iPSCs). Using those cells, we established a co-culture system to study HBV infection and replication in vitro.
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