Titlebook: Hepatitis C Protocols; Johnson Yiu-Nam Lau Book 1998 Springer Science+Business Media New York 1998 infectious diseases

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Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCRolecular techniques. Since archived formalin-fixed, paraffin embedded tissues from these patients are readily available for retrospective studies, development of an assay for the detection of HCV RNA in these fixed tissues will be useful for studying HCV pathogenesis and the effect of interferon-α therapy.

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Molecular Evolutionary Analysisding of the fundamental principles of molecular evolution. Unfortunately, inexperienced investigators are also prone to draw inappropriate or wrong conclusions from this type of analysis. In particular, extreme caution should be taken when phylogenetic trees are applied to clinical settings.

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The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNAinally, in cases of acute HCV infection resulting from accidental needlestick exposure, many patients fail to produce antibody to HCV (.), which makes diagnosis of HCV infection impossible using immunoserological techniques. At the present time, an immunological assay for direct detection of HCV ant

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Hepatitis C Virustructural or nonstructural proteins or a region as short as 222 nt of NS5B. On the basis of such comparisons, it is possible to differentiate consistently among six major genotypes (.), and to an increasing number of subtypes (.), now exceeding 10 for types 1, 2, 3, 4, and 6, but with only two known

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Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Regionble to determine all these genotypes by one method. However, the prevalence of HCV genotypes has been well studied in some countries, including Japan and the United States (.–.). Detection of genotypes by polymerase chain reaction (PCR) using type-specific primers or probes is useful in this area wh

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