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Titlebook: Hepatic Stellate Cells; Methods and Protocol Ralf Weiskirchen,Scott L. Friedman Book 2023 The Editor(s) (if applicable) and The Author(s),

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发表于 2025-3-21 18:05:44 | 显示全部楼层 |阅读模式
书目名称Hepatic Stellate Cells
副标题Methods and Protocol
编辑Ralf Weiskirchen,Scott L. Friedman
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Hepatic Stellate Cells; Methods and Protocol Ralf Weiskirchen,Scott L. Friedman Book 2023 The Editor(s) (if applicable) and The Author(s),
描述This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism. The book features methods to isolate, evaluate, and manipulate this cell type in an effort to elucidate hepatic biology and establish prospects for treating disease. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Hepatic Stellate Cells: Methods and Protocols. serves as an ideal aid to help researchers accelerate the outstanding science that has steadily unveiled the mysteries of stellate cell biology and their role in disease..
出版日期Book 2023
关键词Liver cell biology; Stellate cell isolation; Disease treatment; Extracellular matrix; Homeostasis; Immuno
版次1
doihttps://doi.org/10.1007/978-1-0716-3207-9
isbn_softcover978-1-0716-3209-3
isbn_ebook978-1-0716-3207-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells, cells (SECs), and hepatic stellate cells (HSCs). Normally, in their quiescent state, HSCs are a reservoir for vitamin A, but in response to hepatic injury, they become activated myofibroblasts that play a key role in the hepatic fibrotic response. Activated HSCs express extracellular matrix (ECM) p
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Phalloidin Staining for F-Actin in Hepatic Stellate Cells, contractile, α-smooth muscle actin-positive myofibroblast. These cells acquire properties that are strongly associated with the reorganization of the actin cytoskeleton. Actin possesses a unique ability to polymerize into filamentous actin (F-actin) form its monomeric globular state (G-actin). F-ac
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Retinyl Ester Analysis by Orbitrap Mass Spectrometry, of retinyl ester species by high-resolution mass spectrometry. Retinyl esters are extracted by the method of Bligh and Dyer and subsequently separated by HPLC in runs of 40 min. The retinyl esters are identified and quantified by mass spectrometry analysis. This procedure enables the highly sensiti
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Isolation of Hepatic Stellate Cells and Lymphocytes for Co-culture Systems,unt of extracellular matrix such as collagen fibers. However, recent lines of evidence have also highlighted the immunoregulatory functions of HSCs, in which they interact with diverse hepatic lymphocytes to produce cytokines and chemokines, release extracellular vesicles, or express specific ligand
发表于 2025-3-23 01:00:20 | 显示全部楼层
Working with Immortalized Hepatic Stellate Cell Lines,ved considerable attention in studies investigating fundamental features of hepatic fibrosis. However, the limited supply and ever-increasing demand for these cells, combined with the additional tightening of formal standards in animal welfare policy, make working with these primary cells increasing
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Induction of Obstructive Cholestasis in Mice,eriportal biliary fibrosis. These changes are time-dependent and based on excess accumulation of bile acids in the liver. This in turn causes damage of hepatocytes and functional loss, leading to recruitment of inflammatory cells. Liver resident pro-fibrogenic cells facilitate extracellular matrix s
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Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation, not available. Accordingly, there is a strong need to conduct intensive basic research, which also includes the necessity to use animal models to evaluate new anti-fibrotic therapy concepts. Numerous mouse models of liver fibrogenesis have been described. This involves chemical, nutritional, surgic
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