Titlebook: Hemorrhagic Fever Viruses; Methods and Protocol Maria S. Salvato Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 20

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An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Virusesurveillance for viruses of a specific taxonomic group provides the investigator with sequence files from cDNA amplified using specific primers to conserved viral regions. Here we will describe general approaches to identify hemorrhagic viral agents through phylogenetic analysis of cDNA sequences obtained during surveillance activities.

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Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

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Structure–Function Assays for Crimean–Congo Hemorrhagic Fever Virus Polymerases and measuring the effects these mutations or treatments have on viral life cycle stages. Importantly, these systems can be used at low-containment levels. Presented are the materials and methods currently available to study CCHFV transcription, replication, and translation in the context of a minigenome or VLP.

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Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NPs) in Viral RNA Synthesis and in Supthe role of NP in replicating and transcribing a viral promoter-driven luciferase reporter gene. These powerful assays demonstrate the versatility of NP in mediating viral replication as well as in modulating host innate immune responses.

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Volatile Compounds of Royal Jellyion method. We found that the volatile fraction of RJ is composed of different compounds such as carboxylic acids (2-methylbutanoic acid, 3-methylbutanoic acid, pentanoic acid, 2-hexenoic acid…), phenols (hydroquinone), carbonyl compounds (ketones), esters, aldehydes and alcohols. In this chapter, b