书目名称 | Handbook of ELISPOT | 副标题 | Methods and Protocol | 编辑 | Alexander E. Kalyuzhny | 视频video | | 概述 | Extends and expands upon the hands-on methodologies of the popular first edition.Features expert techniques ready for the laboratory.Includes key tips and implementation advice to ensure successful re | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of .Handbook of ELISPOT: Methods and Protocols., only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and | 出版日期 | Book 2012Latest edition | 关键词 | Animal models; Cell research; Diagnostics; Enzyme-linked immunospot assay; Image analysis; Immune system | 版次 | 2 | doi | https://doi.org/10.1007/978-1-61779-325-7 | isbn_softcover | 978-1-4939-6111-5 | isbn_ebook | 978-1-61779-325-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2012 |
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