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Titlebook: HIV Protocols; Nelson L. Michael,Jerome H. Kim Book 19991st edition Humana Press 1999

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https://doi.org/10.1007/978-3-642-59246-1he core promoter elements by the viral Tat protein is located within the R region of the LTR at positions +1 to +59 (.,.). This transactivating region (TAR) folds into alternate RNA stem-loop structures that are found in the 5′ termini of all viral transcripts. The TAR element mediates a substantial
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Methods in Molecular Medicinehttp://image.papertrans.cn/h/image/420198.jpg
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culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.
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Quantitative HIV Culture culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.
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https://doi.org/10.1007/978-3-030-21784-6The predominant mode of transmission of HIV-1 worldwide is sexual intercourse. Therefore, there has been growing interest in studying HIV-1 in genital secretions. Given the urgency to develop a vaccine to protect against HIV-1, techniques have been developed to isolate and quantitate HIV-1 from genital secretions.
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Christina Hajisoteriou,Panayiotis AngelidesA salient feature regarding the HIV (and all the retroviruses in general) replicative cycle is the retrotranscription of the RNA genome to DNA (provirus), followed by its integration in the genome of the host cell.
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Methods of Culturing HIV-1 from SemenThe predominant mode of transmission of HIV-1 worldwide is sexual intercourse. Therefore, there has been growing interest in studying HIV-1 in genital secretions. Given the urgency to develop a vaccine to protect against HIV-1, techniques have been developed to isolate and quantitate HIV-1 from genital secretions.
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Quantitative PCR for HIV-1 Proviral DNAA salient feature regarding the HIV (and all the retroviruses in general) replicative cycle is the retrotranscription of the RNA genome to DNA (provirus), followed by its integration in the genome of the host cell.
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