Titlebook: HIV Protocols; Second Edition Vinayaka R. Prasad,Ganjam V. Kalpana Book 2009Latest edition Humana Press 2009 AIDS vaccine.Antiretrovirals.H

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书目名称HIV Protocols影响因子(影响力)学科排名




书目名称HIV Protocols网络公开度




书目名称HIV Protocols网络公开度学科排名




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书目名称HIV Protocols被引频次学科排名




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书目名称HIV Protocols读者反馈学科排名

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1064-3745 for their expertise in the respective methodologies.Covers b.Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets a

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Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomo produce cDNA copies, and PCR amplification of long HIV-1 gene fragments using site-specific, overlapping primers. The primers are based on subtype B HIV-1 strains, and plasma specimens are used in the procedures. However, the protocol can easily be adapted to other HIV-1 subtypes by modifying the primers to match the subtype of interest.

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Vinayaka R. Prasad,Ganjam V. KalpanaComprehensive account of cutting-edge, virological techniques with a wide range of protocols.Written by well-known HIV researchers selected for their expertise in the respective methodologies.Covers b

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Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/420197.jpg

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HIV Protocols978-1-59745-170-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomluids therefore reflect the virus present in real time. To obtain near full-length genomic sequences derived from virion RNA it is first necessary to carefully isolate and amplify the RNA..The procedure described below, involves viral RNA extraction, reverse transcription (RT) of the extracted RNA t

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Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemictroviruses can be complicated by the contamination of even highly purified virion preparations with nonviral particles (either microvesicles or exosomes). Two useful methods have been developed that can remove contaminating particles from virus stocks to produce highly pure virus preparations. One a