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Titlebook: HDAC/HAT Function Assessment and Inhibitor Development; Methods and Protocol Oliver H. Krämer Book 2017 Springer Science+Business Media New

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发表于 2025-3-21 17:09:57 | 显示全部楼层 |阅读模式
书目名称HDAC/HAT Function Assessment and Inhibitor Development
副标题Methods and Protocol
编辑Oliver H. Krämer
视频videohttp://file.papertrans.cn/421/420177/420177.mp4
概述Includes cutting-edge methods and protocols to study HDACs and HATs.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includ
丛书名称Methods in Molecular Biology
图书封面Titlebook: HDAC/HAT Function Assessment and Inhibitor Development; Methods and Protocol Oliver H. Krämer Book 2017 Springer Science+Business Media New
描述This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols. serves as an ideal guide to this vital area of study..
出版日期Book 2017
关键词Reversible protein acetylation; Deacetylase inhibitors; Histone deacetylases; Histone acetyltransferase
版次1
doihttps://doi.org/10.1007/978-1-4939-6527-4
isbn_softcover978-1-4939-8226-4
isbn_ebook978-1-4939-6527-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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发表于 2025-3-21 23:31:42 | 显示全部楼层
https://doi.org/10.1007/978-1-4020-4968-2 the response of the immune system, like the B cell function. HDACi treatment affects differentiation, proliferation, and survival of B cells. Here we describe how to investigate the effects of HDACi treatment on naïve B cells regarding class-switch recombination (CSR) in vitro using flow cytometry.
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https://doi.org/10.1057/978-1-137-56104-6 lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.
发表于 2025-3-22 05:57:29 | 显示全部楼层
https://doi.org/10.1007/978-3-031-29746-5heir ability to target and kill cancer cells in vivo. Here, we describe the xenografting of (germ cell) cancer cell lines into the flank of nude mice and the subsequent intravenous application of HDIs.
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Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Ho lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.
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发表于 2025-3-23 05:06:44 | 显示全部楼层
Oliver H. KrämerIncludes cutting-edge methods and protocols to study HDACs and HATs.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includ
发表于 2025-3-23 06:04:43 | 显示全部楼层
Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/420177.jpg
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