书目名称 | Fluorescent Protein-Based Biosensors |
副标题 | Methods and Protocol |
编辑 | Jin Zhang,Qiang Ni,Robert H Newman |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .In .Fluorescent Protein-Based Biosensors: Methods and Protocols., experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics,. .lists of the necessary materials and reagents, step-by-step, readily reproducible. .protocols, and notes on troubleshooting and avoiding known pitfalls.. .Authoritative and easily accessible, .Fluorescent Protein-Based Biosensors: Methods and Protocols .promises to be a valuable resource for researchers. .interested in applying |
出版日期 | Book 2014 |
关键词 | FRET indicators; biosensors; fluorescent proteins; kinase activity; live cell imaging |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-62703-622-1 |
isbn_softcover | 978-1-4939-6014-9 |
isbn_ebook | 978-1-62703-622-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2014 |