书目名称 | Fluorescence In Situ Hybridization (FISH) | 副标题 | Application Guide | 编辑 | Thomas Liehr | 视频video | | 概述 | Offers readily reproducible, step-by-step laboratory methods.Features easy-to-follow recipes to promote successful results.Includes sections on interpreting and evaluating results, as well as troubles | 丛书名称 | Springer Protocols Handbooks | 图书封面 |  | 描述 | .This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. .This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working inthe fields of human genetics, microbiology, animal and plant sciences.. | 出版日期 | Book 2017Latest edition | 关键词 | Chromosomes; Molecular cytogenetic diagnostics; Multicolor FISH; Immunostaining; Microdissection; Fluores | 版次 | 2 | doi | https://doi.org/10.1007/978-3-662-52959-1 | isbn_softcover | 978-3-662-57100-2 | isbn_ebook | 978-3-662-52959-1Series ISSN 1949-2448 Series E-ISSN 1949-2456 | issn_series | 1949-2448 | copyright | Springer-Verlag Berlin Heidelberg 2017 |
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