| 书目名称 | Fluorescence In Situ Hybridization (FISH) | | 副标题 | Methods and Protocol | | 编辑 | Gal Haimovich | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting edge and thorough, .Fluorescence In Situ Hybridization (FISH): Methods and Protocols. is a valuable resource that will benefit the broader scientific commu | | 出版日期 | Book 2024 | | 关键词 | mRNA; immunofluorescence; DNA FISH; CRISPR; single-nucleotide polymorphisms (SNPs) | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-3766-1 | | isbn_softcover | 978-1-0716-3768-5 | | isbn_ebook | 978-1-0716-3766-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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发表于 2025-3-21 16:42:56
