书目名称 | Fluorescence Correlation Spectroscopy |
副标题 | Theory and Applicati |
编辑 | Rudolf Rigler,Elliot S. Elson |
视频video | http://file.papertrans.cn/345/344700/344700.mp4 |
概述 | First book on this important analytical technique |
丛书名称 | Springer Series in Chemical Physics |
图书封面 |  |
描述 | Fluorescence correlation spectroscopy (FCS) was developed in order to char acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea surements of spontaneous microscopic thermally driven molecular concentra tion fluctuations. Since its inception, and particularly in recent years, techni cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic conce |
出版日期 | Book 2001 |
关键词 | Fluorescence correlation microscopy; Single molecule analysis; Thermodynamic fluctuations; diffraction; |
版次 | 1 |
doi | https://doi.org/10.1007/978-3-642-59542-4 |
isbn_softcover | 978-3-642-64018-6 |
isbn_ebook | 978-3-642-59542-4Series ISSN 0172-6218 Series E-ISSN 2364-9003 |
issn_series | 0172-6218 |
copyright | Springer-Verlag Berlin Heidelberg, New York 2001 |