书目名称 | Fibrosis | 副标题 | Methods and Protocol | 编辑 | Laure Rittié | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume describes state-of-the-art protocols that serve as “recipes” for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Cutting-edge and practical, .Fibrosis: Methods and Protocols .is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.. | 出版日期 | Book 2017 | 关键词 | bleomycin; Myocardial infarction; fibroblasts; mesenchymal stem cells; adipose; cell culture systems; ECM | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-7113-8 | isbn_softcover | 978-1-4939-8388-9 | isbn_ebook | 978-1-4939-7113-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media LLC 2017 |
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