书目名称 | Expressed Sequence Tags (ESTs) | 副标题 | Generation and Analy | 编辑 | John Parkinson | 视频video | | 概述 | Essential reading for any scientist beginning their own EST project.Takes the reader through the entire process of how to successfully complete an EST project.Covers a wide range of cDNA library const | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In .Expressed Sequence Tags (ESTs): Generation and Analysis., leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls...Cutting-edge and easy to use, .Expressed Sequence Tags (ESTs): Gener | 出版日期 | Book 2009 | 关键词 | Alternative transcripts; Bur; Expression profiling; Gene discovery; Genetic diversity; Microarray; Phyloge | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60327-136-3 | isbn_softcover | 978-1-61737-760-0 | isbn_ebook | 978-1-60327-136-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2009 |
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