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Titlebook: Expressed Sequence Tags (ESTs); Generation and Analy John Parkinson Book 2009 Humana Press 2009 Alternative transcripts.Bur.Expression prof

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发表于 2025-3-21 16:27:02 | 显示全部楼层 |阅读模式
书目名称Expressed Sequence Tags (ESTs)
副标题Generation and Analy
编辑John Parkinson
视频video
概述Essential reading for any scientist beginning their own EST project.Takes the reader through the entire process of how to successfully complete an EST project.Covers a wide range of cDNA library const
丛书名称Methods in Molecular Biology
图书封面Titlebook: Expressed Sequence Tags (ESTs); Generation and Analy John Parkinson Book 2009 Humana Press 2009 Alternative transcripts.Bur.Expression prof
描述.Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In .Expressed Sequence Tags (ESTs): Generation and Analysis., leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls...Cutting-edge and easy to use, .Expressed Sequence Tags (ESTs): Gener
出版日期Book 2009
关键词Alternative transcripts; Bur; Expression profiling; Gene discovery; Genetic diversity; Microarray; Phyloge
版次1
doihttps://doi.org/10.1007/978-1-60327-136-3
isbn_softcover978-1-61737-760-0
isbn_ebook978-1-60327-136-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
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发表于 2025-3-21 23:30:16 | 显示全部楼层
Strategies for Undertaking Expressed Sequence Tag (EST) Projects,rposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly define the project purpose, inclu
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Preparation of Full-Length cDNA Libraries: Focus on Metazoans,btraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtra
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Generating EST Libraries: ,-Spliced cDNAs,r a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates, as well as members from five metazoan phyla: Cnidaria, Rotifera, Nematoda, Platyhelminths and Chordat
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Large-Scale Sequencing and Analytical Processing of ESTs,, their multipurpose uses, such as in probe design for microarrays, determining alternative splicing, verifying open reading frames, and confirming exon/intron and gene boundaries, to name a few, further justify their inclusion in many genomic characterization projects. Hence, there has been a const
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EST Processing: From Trace to Sequence,bjects. Here we describe the usage of a software pipeline (available from .), which has been developed to make the most of EST datasets. This modular software solution is targeted toward small- to medium-sized EST projects and comprises a series of Perl scripts. The software design is based on our e
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