| 书目名称 | Exon Skipping |
| 副标题 | Methods and Protocol |
| 编辑 | Annemieke Aartsma-Rus |
| 视频video | |
| 概述 | Provides an overview of the exon skipping field.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary mat |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from .in vitro. splicing to .in vivo. tests in animal models requiring a more extensive set of protocols. .Exon Skipping: Methods and Protocols. provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical. Exon Skipping: Methods and Protocols. seeks to aid scientists in the continuing study of exon skipping.. |
| 出版日期 | Book 2012 |
| 关键词 | Exon Skipping; RNA; in vitro splicing; in vivo tests; mRNA; multiple genes; mutations; next generation sequ |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-61779-767-5 |
| isbn_softcover | 978-1-4939-6206-8 |
| isbn_ebook | 978-1-61779-767-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media,LLC 2012 |
发表于 2025-3-21 16:34:48
