| 书目名称 | Ewing Sarcoma | | 副标题 | Methods and Protocol | | 编辑 | Florencia Cidre-Aranaz,Thomas G. P. Grünewald | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise i.n vitro .techniques including re-expression and functional analyses of target genes together with .in vivo. tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma. A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Cutting-edge and clearly written, .Ewing sarcoma: Methods and Protocols. aims to provide methods to further the study | | 出版日期 | Book 2021 | | 关键词 | Epigenetic alterations; FISH; qRT-PCR; RNA and DNA sequencing; zebrafish | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-1020-6 | | isbn_softcover | 978-1-0716-1022-0 | | isbn_ebook | 978-1-0716-1020-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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发表于 2025-3-21 17:26:27
