Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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https://doi.org/10.1007/978-3-031-44995-6knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative concentration-response information on estrogenic activity of chemical compounds.

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https://doi.org/10.1007/978-3-031-44294-0lational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estro

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Afia Akhter Lipi,Yukiko Nakano,Matthias Rehmssion to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of two estrogen receptors, both isoforms of the genomic estro

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