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Titlebook: Estimation of Microbial Protein Supply in Ruminants Using Urinary Purine Derivatives; Harinder P. S. Makkar,X. B. Chen Conference proceedi

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Measurements of Purine Derivatives and Creatinine in Spot Urine Samples of Chinese Yellow Cattlebased rations would improve rumen microbial protein synthesis. Within each farm, the animals were fed on five diets. Diets 1–2 were typical rice straw + by-products used by farmers and were low in fermentable energy content; Diets 3–5 were more balanced, containing a higher content of fermentable en
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Estimation of Microbial Protein Supply of Lactating Dairy Cows under Smallholder Farms in North-Eastt livestock under farm conditions. Results of Experiment 1 indicated that diurnal variation in the PDC index ( [mmol/L PD]/[mmol/L creatinine] kgW.) in spot urine samples of zebu cattle was small and highly correlated with the daily PD output, suggesting that spot urine samples could be used to deri
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Purine Derivatives/Creatinine Ratio as an Index of Microbial Protein Synthesis in Lactating Holsteinion as an index of microbial N synthesized in the rumen. In the first experiment, differences in creatinine determination method were studied: creatinine excretion was 26.8 ±1.2 and 27.4 ±2.2 mg/kg live weight, or 1,175.39 ±74.69 and 1,199.9 ±103.9 mmol/kgW. respectively for HPLC and colorimetry ana
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Urinary Excretion of Purine Derivatives and Its Utilization as an Index of Microbial Protein Synthesing the excretion rate of purine derivatives (PD) in urine. In Experiment I, a significant linear regression was established between the level of feed intake and the urinary excretion of total PD. The amount of PD excretion in urine increased by about 11 mmol PD/kg digestible organic matter intake/d
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Measurement of Purine Derivatives and Creatinine in Urine by HPLC described. PD separation and quantification were achieved using two Spherisorb ODS 2 reversed phase columns connected in series (4.6 × 250 mm) and a Spherisorb ODS 2 Waters pre-column and a Perkin Elmer pump with an auto sampler. The mobile phase was NH.H.PO.:NH.H.PO.-acetonitrile (80:20), which wa
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