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Titlebook: Epigenome Editing; Methods and Protocol Albert Jeltsch,Marianne G. Rots Book 2018 Springer Science+Business Media, LLC, part of Springer Na

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发表于 2025-3-21 19:03:02 | 显示全部楼层 |阅读模式
书目名称Epigenome Editing
副标题Methods and Protocol
编辑Albert Jeltsch,Marianne G. Rots
视频video
概述Includes cutting-edge techniques for the study of epigenome editing.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epigenome Editing; Methods and Protocol Albert Jeltsch,Marianne G. Rots Book 2018 Springer Science+Business Media, LLC, part of Springer Na
描述This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Epigenome Editing: Methods and Protocols. will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved..
出版日期Book 2018
关键词Histone protein modifications; CRISPR; dCas9; EpiEditors; DNA-binding device; Epigenome reprogramming
版次1
doihttps://doi.org/10.1007/978-1-4939-7774-1
isbn_softcover978-1-4939-9281-2
isbn_ebook978-1-4939-7774-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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An Anthropological Study of Hospitalitypatiotemporal control. Nearly any brain region of interest can be targeted in rodents at every stage of postnatal life. Owing to the versatility, reproducibility, and utility of this technique, it is an important method for any laboratory interested in studying the cellular, circuit, and behavioral consequences of in vivo neuroepigenome editing.
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Social Designation of Cultural Markers,rget the epitope of interest. The DNA is released from the proteins by heat and protease treatment, followed by degradation of contaminating RNAs with RNase. The resulting DNA is analyzed using various methods, including PCR, qPCR, or sequencing. This protocol outlines each of these steps for both yeast and human cells.
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Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniquespatiotemporal control. Nearly any brain region of interest can be targeted in rodents at every stage of postnatal life. Owing to the versatility, reproducibility, and utility of this technique, it is an important method for any laboratory interested in studying the cellular, circuit, and behavioral consequences of in vivo neuroepigenome editing.
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