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Titlebook: Epigenetic Reprogramming During Mouse Embryogenesis; Methods and Protocol Katia Ancelin,Maud Borensztein Book 2021 Springer Science+Busines

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书目名称Epigenetic Reprogramming During Mouse Embryogenesis
副标题Methods and Protocol
编辑Katia Ancelin,Maud Borensztein
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epigenetic Reprogramming During Mouse Embryogenesis; Methods and Protocol Katia Ancelin,Maud Borensztein Book 2021 Springer Science+Busines
描述.This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols. is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming. .
出版日期Book 2021
关键词PGCs; pluripotence acquisition; CRISPR; 3D DNA FISH; embryo transcriptome
版次1
doihttps://doi.org/10.1007/978-1-0716-0958-3
isbn_softcover978-1-0716-0960-6
isbn_ebook978-1-0716-0958-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Begriff des „abhängig“ Erwerbstätigene three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the sample processing, protein detection using antibodies and confocal imaging of isolated preimplantation to early postimplantation mouse embryos up to Embryonic day 8.0 (E8.0).
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,Approximations- und Symmetrisierungssätze,an explore methylation differences between cells in mixed cell populations and profile methylation in very rare cell types, such as mammalian oocytes and cells from early embryos. Here, we outline the scBS-seq protocol in a 96-well plate format applicable to studies of moderate throughput.
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Whole-Mount Immunofluorescence Staining of Early Mouse Embryos,e three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the sample processing, protein detection using antibodies and confocal imaging of isolated preimplantation to early postimplantation mouse embryos up to Embryonic day 8.0 (E8.0).
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Profiling DNA Methylation Genome-Wide in Single Cells,an explore methylation differences between cells in mixed cell populations and profile methylation in very rare cell types, such as mammalian oocytes and cells from early embryos. Here, we outline the scBS-seq protocol in a 96-well plate format applicable to studies of moderate throughput.
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Otto Blume,Wolfgang Plum,Gerhard Naegelecessible to experimental and genetic manipulations, its normal or perturbed development can be scrutinized ex vivo by real-time imaging from fertilization to late blastocyst stage. High-resolution imaging of multiple embryos at the same time can be compromised by embryos displacement during imaging.
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