Titlebook: Enzymes of Molecular Biology; Michael M. Burrell Book 1993 Humana Press 1993

Figate

convert

橡子

Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1),ses that preferentially catalyze the hydrolysis of double-stranded DNA (ds DNA) and that have found a use in the various techniques employed in molecular biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are

laparoscopy

遗留之物

Dendritic-Cells

Reverse Transcriptase (EC 2.7.7.49), first in this process, retroviral RNA-directed DNA polymerase (reverse transcriptase), catalyzes the synthesis of a DNA copy of an RNA template in the presence of a suitable primer. Reverse transcriptase (RT) was discovered in 1970 (.,.) and was first used to copy a eukaryotic cell mRNA in 1971 (.–

oxidize

Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31),ynucleotide primers. Different from all the other known DNA polymerases, TdT-catalyzed DNA synthesis is not template-directed. The reaction can be described as: . Only one dNTP is necessary for polymerization, although any dNTP (and derivatives thereof) or any combination of dNTPs will serve as subs

nonradioactive

Restriction Enzymes, identified in many prokaryotic organisms and are considered to be part of a defense system directed against foreign DNA. Site-specific endodeoxyribonucleases are given EC numbers, e.g., EC 3.1.21.4 for a Type II enzyme ..

diskitis