Titlebook: Enzyme-Mediated Ligation Methods; Timo Nuijens,Marcel Schmidt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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Le Carré Landscapes: The Cold Warheterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.

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Fabiola H. Gerpott,Alfred Kiesere describe a protocol to perform SPOCQ reaction on proteins, along with notes to optimize yield and reaction rates. Conjugation efficiencies of over 95% to antibodies have been reported using this protocol.

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Enzyme-Mediated Ligation Methods978-1-4939-9546-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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After-Sales-Feedback mit Kundenkonferenzeng an LPXTG SrtA recognition motif. Unfortunately, SrtA suffers from low turnover rates, resulting in poor ligation efficiencies even with optimized reaction conditions. In this chapter, we describe proximity-based sortase-mediated ligation (PBSL), which uses the SpyTag–SpyCatcher peptide–protein pai