书目名称 | Enzyme Stabilization and Immobilization | 副标题 | Methods and Protocol | 编辑 | Shelley D. Minteer | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and t | 出版日期 | Book 2017Latest edition | 关键词 | liposome formation; micelle introduction; crosslinking; sol-gel encapsulation; polymer encapsulation; sin | 版次 | 2 | doi | https://doi.org/10.1007/978-1-4939-6499-4 | isbn_softcover | 978-1-4939-8219-6 | isbn_ebook | 978-1-4939-6499-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 2017 |
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