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Titlebook: Enzyme Stabilization and Immobilization; Methods and Protocol Shelley D. Minteer Book 2017Latest edition Springer Science+Business Media Ne

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书目名称Enzyme Stabilization and Immobilization
副标题Methods and Protocol
编辑Shelley D. Minteer
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Enzyme Stabilization and Immobilization; Methods and Protocol Shelley D. Minteer Book 2017Latest edition Springer Science+Business Media Ne
描述.This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and t
出版日期Book 2017Latest edition
关键词liposome formation; micelle introduction; crosslinking; sol-gel encapsulation; polymer encapsulation; sin
版次2
doihttps://doi.org/10.1007/978-1-4939-6499-4
isbn_softcover978-1-4939-8219-6
isbn_ebook978-1-4939-6499-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
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Francis E. Godwyll,Siphokazi Magadlay applications, the goal is to provide extended active lifetime at normal environmental conditions with traditional substrates at low concentrations in buffered solutions. However, as enzymes are used for more and more applications, there is a desire to use them in extreme environmental conditions (
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African Climate and Climate Change, as well as the pH and solvent tolerance of enzymes. This field is traditionally referred to as micellar enzymology. This chapter details the use of ionic and nonionic micelles for the stabilization of polyphenol oxidase, lipase, and catalase, although this method could be used with any enzymatic s
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What is African Communitarianism?,erwhelming results. Enzymes are frequently inactivated in RTILs as a result of the influence of solvent on the enzyme’s microenvironment, be it through interacting with the enzyme or enzyme-bound water molecules. The purpose of this chapter is to present a rational approach to mediate RTIL–enzyme in
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https://doi.org/10.1007/978-3-319-32017-5area form of gold that is suitable for physisorption or for covalent modification by self-assembled monolayers. The material can be used as a high surface area electrode and with immobilized enzymes can be used for amperometric detection schemes. NPG can be prepared in a variety of formats from allo
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Privacy and Data Protection in Lesothotivity compared to soluble molecules and the material simplifies catalyst integration compared to other methods. A silica sol-gel process based upon biological mechanisms of inorganic material formation (biomineralization) supports protein immobilization reactions within minutes. The material has hi
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