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Titlebook: Enzymatic Analysis; A Practical Guide Janet V. Passonneau,Oliver H. Lowry Book 1993 Springer Science+Business Media LLC 1993 enzyme.enzymes

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发表于 2025-3-21 19:13:28 | 显示全部楼层 |阅读模式
书目名称Enzymatic Analysis
副标题A Practical Guide
编辑Janet V. Passonneau,Oliver H. Lowry
视频video
丛书名称Biological Methods
图书封面Titlebook: Enzymatic Analysis; A Practical Guide Janet V. Passonneau,Oliver H. Lowry Book 1993 Springer Science+Business Media LLC 1993 enzyme.enzymes
描述Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purpo
出版日期Book 1993
关键词enzyme; enzymes; NADP; Nucleotide
版次1
doihttps://doi.org/10.1007/978-1-60327-407-4
isbn_softcover978-1-4899-4014-8
isbn_ebook978-1-60327-407-4
copyrightSpringer Science+Business Media LLC 1993
The information of publication is updating

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发表于 2025-3-21 22:45:44 | 显示全部楼层
https://doi.org/10.1007/978-3-030-78083-8A rudimentary working knowledge of enzyme kinetics is of great value in the use of enzymes as analytical tools. An analysis may fail if too little enzyme is used, because of incomplete reaction, but it may also fail if too much enzyme is used. This might not be true if absolutely pure, single-action, enzymes were available.
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A Kinetics Primer for the Tissue AnalystA rudimentary working knowledge of enzyme kinetics is of great value in the use of enzymes as analytical tools. An analysis may fail if too little enzyme is used, because of incomplete reaction, but it may also fail if too much enzyme is used. This might not be true if absolutely pure, single-action, enzymes were available.
发表于 2025-3-22 13:03:54 | 显示全部楼层
Improvement, Modification, Adaptation, Troubleshooting, and Development of New MethodsThis chapter is intended to offer suggestions for simplifying or improving methods, for adapting methods to a different order of sensitivity, and for developing new methods. It also discusses some of the difficulties that may arise and ways to circumvent them.
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Bijal Panchal,Andrea Bagdadi,Ipsita Roybed a method for the determination of glucose-6-P dehydrogenase activity based on the increase in absorption in the near UV as NADPH was produced. This is apparently the first publication of a method of this type. Greengard (.) was the first to describe fluorometric pyridine nucleotide methods for m
发表于 2025-3-23 02:55:32 | 显示全部楼层
Yuri A. Novikov,Vasiliy V. Novohatin made (.) and the ease of use make them particularly valuable for microchemical analyses. In fact, for all of the analytical applications of the type considered in this book, the most important tool is the constriction pipet. The construction, calibration, and use of this instrument therefore deserv
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