Titlebook: Environmental Responses in Plants; Methods and Protocol Paula Duque,Dóra Szakonyi Book 2022Latest edition The Editor(s) (if applicable) and

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Imaging with a 2D Transducer Hybrid Arrayd ribosome distribution on mRNA can be gathered by carrying out ribosome footprinting, aka Ribo-seq. Herein, we present a detailed protocol describing the construction of parallel Ribo-seq and RNA-seq libraries from . seedlings treated with the plant hormone auxin. The improved protocol for ribosome

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https://doi.org/10.1007/978-1-4684-3755-3tyl length is mainly used in phytochrome physiological assays to determine the functional impact of photoreceptor point mutations, different intracellular localizations, or the function of signal transduction components, phytochrome-controlled seed germination offers a different, very sensitive tool

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John M. Richardson,Glen Wade,George Goebeldestructive, and non-transgenic assay of plant circadian rhythms that can be readily automated, greatly facilitating genetic studies. Rhythmic leaf movement is particularly useful for the assessment of standing variation in clock function and can be readily applied to a diverse array of dicotyledono

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Three-Dimensional Acoustic Imagingdetermining changes in the composition of photosynthetic pigments (chlorophyll and carotenoids), and we also consider its implementation within circadian experiments. We describe how to design a circadian experiment with the goal of assessing changes in pigment composition. We then perform two conse

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Imaging Acoustics and Acoustic Imagingle in chloroplasts, such as in the regulation of photosynthetic enzymes or the transition from light to dark. Only recently, the underlying molecular machinery, e.g., transporters and channels that enable chloroplast Ca. fluxes, has started to be elucidated. However, chemical tools to specifically p

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Sidney Leeman,Mark Betts,Leonard Ferraring social and economic impacts. Understanding rice responses to stress may guide breeding for more tolerant varieties. However, the lack of consistency in the design of the stress experiments described in the literature limits comparative studies and output assessments. The use of identical setups i

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Jafar Saniie,Tao Wang,Nihat M. Bilgutayenotypes at the early plant development stages is fundamental to identify new regulators of the ABA pathway, which in turn is extremely relevant for agriculture in a global climate change context. Typically, under experimental conditions, seeds are germinated in hormone-containing plates, and postge

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M. Krueger,A. Pesavento,H. Ermertto stress that allow them to gain time while mounting a major response. This first line of defense includes the stomata, leaf epidermal pores in charge of regulating water loss and photosynthesis. Stomatal movements are controlled by the stress phytohormone abscisic acid (ABA), which induces fast cl