Titlebook: Environmental Monitoring of Bacteria; Clive Edwards Book 1999 Humana Press 1999

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Analysis of DNA Sequences,ce of target organisms. In addition, fluorescently labeled olignucleotides are increasingly being used in combination with flow cytometry for cell counting or sorting (.,.), and with confocal laser scanning microscopy (.,.) to generate . data revealing close spatial associations of organisms in envi

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Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology,ariation in the targeted nucleotide sequences. Nucleotide pair dissociation is mediated in denaturing gradient gels (DGGE) by the chemicals urea and formamide. These denaturants are incorporated into the gel in increasing concentrations to form the denaturing gradient. A so-called melting domain wit

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Reporter Gene Expression for Monitoring Microorganisms in the Environment,ular organism or to monitor its activity. The reporter gene can be located on a plasmid or to increase the stability of the gene within the cell it can be incorporated into the bacterial chromosome. There are a wide variety of reporter genes available, and all have particular properties that make th

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Fluorescent , Hybridization and the Analysis of the Single Cell,ered as mosaics of highly conserved and highly variable sequence. Regions of highly conserved sequence have remained virtually unchanged throughout evolution and provide ideal targets for so-called universal or consensus probes and for probes directed at higher levels of taxonomic rank. The variable

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https://doi.org/10.1057/9780230504837. Unfortunately, it is often the case that experimental protocols developed for model species are not transposable to other bacteria. This gulf is often most apparent when deductions made from organisms grown as pure cultures in the laboratory are assumed to apply to those occurring in natural envir