Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut

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ative ,matin ,roteomics (N-ChroP) to Characterize Histone Post-translational Modification (PTM) ComChIP) protocol with the mass spectrometry (MS) analysis of immunoprecipitated proteins to study the combinatorial enrichment or exclusion of histone post-translational modifications (PTMs) at specific genomic regions, such as promoters or enhancers. We describe the protocol steps from the digestion

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Book 2021ers and Promoters: Methods and Protocols. is a useful guide for future experiments..Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

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Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Captureparticularly suited to genome-wide studies of promoter interactions with distal regulatory elements, such as enhancers. We present the principles and methods for Promoter Capture Hi-C (PCHi-C), from experimental design to data analysis.

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The Physical Chemistry of MEMBRANESA interacting complexes ligated and sequenced (RADICL-Seq) a novel methodology to map genome-wide RNA–chromatin interactions in intact nuclei. Here, we describe how CAGE and RADICL-Seq data can be used to characterize enhancer elements and identify their target genes.

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Book 2021scribe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the .Methods in Molecular Biology .series format, chapters include comprehensive introductions, lists of the ne