Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2016 Springer Science+Business Media New York 2016 Cannabis sativa.en

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https://doi.org/10.1007/978-1-4939-3539-0Cannabis sativa; endocannabinoid system; N-arachidonoylethanolamine; 2-arachidonoylglycerol; membrane an

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Reliability of passive electronic parts,bent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen. SureFire. assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by .-α-lysophosphatidylinositol (LPI) and certain cannabinoids.

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U. Dinesh Kumar,John Crocker,M. El-Haram anti-inflammatory and analgesic agents. Here, we introduce an NAAA assay system, using [.C]palmitoylethanolamide and thin-layer chromatography. The preparation of NAAA enzyme from native and recombinant sources as well as the chemical synthesis of .-[1′-.C]palmitoyl-ethanolamine is also described.

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Reliability, Yield, and Stress Burn-Ineoyl[1-.C]-2-arachidonoylglycerol as the substrate is reported. All the steps needed to perform lipid extraction, fractionation by thin-layer chromatography (TLC), and quantification of radiolabeled [.C]-oleic acid via scintillation counting are described in detail.