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Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), u

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Assay of NAT Activity, family (also known as HRAS-like suppressor family) show Ca.-independent NAT activity. In this chapter, we describe (1) partial purification of Ca.-dependent NAT from rat brain, (2) purification of recombinant cPLA.ε and PLAAT-2, and (3) NAT assay using radiolabeled substrate.
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Distribution Models and Functions,e Δ.-tetrahydrocannabinol. During the last 20 years, several .-acylethanolamines and acylesters have been shown to act as eCBs, and a complex array of receptors, metabolic enzymes, and transporters (that altogether form the so-called “eCB system”) has been shown to finely tune their manifold biologi
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https://doi.org/10.1007/978-3-662-54209-5ical conditions. We describe here an analytical protocol for the extraction of endocannabinoids, anandamide (archidonoyl ethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG), and endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic a
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