书目名称 | Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases | 副标题 | Methods and Protocol | 编辑 | Nicole I. Nieden | 视频video | | 概述 | Contains detailed, expert protocols specific to ESCs and stem cell differentiation.Gives special care to address possible methodological pitfalls and provides trouble-shooting guides in the form of No | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.Dependable and cutting-edge, Embryoni | 出版日期 | Book 2011 | 关键词 | Controllable bioreactor processes; Degenerative cartilage & bone diseases; ESCs; Osteoblasts, osteoclas | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60761-962-8 | isbn_softcover | 978-1-4939-5723-1 | isbn_ebook | 978-1-60761-962-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2011 |
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