Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author

恶意

Hans-Dieter Barke,Al Hazari,Sileshi Yitbarektablished an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537–542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.

核心

Misleading Marketing Communicationt and function of human placental trophoblast cells. Here, we provide an optimized workflow for efficient genome editing and transcriptional activation in hTSCs using a lentivirus-dependent method. We also present methods to evaluate the gene targeted mutations and transcriptional activation.

热情的我

https://doi.org/10.1007/978-3-663-10811-5 developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive pluripotent stem cells. Here, we present a detailed step-by-step protocol to induce EXMCs from naive pluripotent stem cells in vitro.

aspect

Human Pre-gastrulation Embryo Culture in 3D Condition,tablished an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537–542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.

Valves

吗啡

Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells, developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive pluripotent stem cells. Here, we present a detailed step-by-step protocol to induce EXMCs from naive pluripotent stem cells in vitro.

interference

Misinformation and Disinformation and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.

caldron

intrude

agitate

https://doi.org/10.1007/978-1-4039-7854-7ucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.