Titlebook: Elispot for Rookies (and Experts Too); Sylvia Janetzki Book 2016 Springer International Publishing Switzerland 2016 B-cell Assay.Elispot.F

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Antecedent

Book 2016nderstand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as

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2367-1114 erstand the best options for specific protocol steps, reagen.This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provid

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Customer Relationship Managementd so perfectly that you end up, for example, with exactly 60 antigen-specific cells in 200,000 cells added to the well, in three consecutive wells). While some variability is acceptable, the question is this: How much variability is acceptable? Let’s take a look at some hypothetical data sets:

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Data Analysis,d so perfectly that you end up, for example, with exactly 60 antigen-specific cells in 200,000 cells added to the well, in three consecutive wells). While some variability is acceptable, the question is this: How much variability is acceptable? Let’s take a look at some hypothetical data sets:

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Reporting Guidelines,erum used pretested? Were reported results background corrected? Or would you like to see an example image of crucial wells (which will likely be the best example available)? Did you wonder how the authors established reading parameters, and if reading parameters were adapted when necessary? Many more questions come to mind.

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Overview,s in parts similar to Elispot, and from the fact that spots are the actual readout of the assay. Elispot has been around for many years. It was first described in 1983 for the detection of heat-labile enterotoxin production of bacteria [1], followed by its use for the enumeration of specific antibod

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