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Titlebook: Electroporation Protocols for Microorganisms; Jac A. Nickoloff Book 1995 Humana Press 1995

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Electrotransformation in ,,mit uptake of DNA at levels adequate for molecular cloning. Transformation frequencies using Ca.-shock methods with selected strains of . K-12 range from 10. to 10. transformants/µg of covalently closed circular plasmid DNA, but efficiency is frequently lower because these methods are very sensitive
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Electrotransformation of ,, organic matter mineralization. Some species can cause diseases of plants, with various host specificities, whereas others are important opportunistic human and animal pathogens. Many species are metabolically diverse, capable of utilizing a variety of organic compounds as the sole source of carbon
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Electroporation of ,,niums. However, . species do not respond well to chemical treatments that induce competence necessary for transformation studies, and a highly reproducible natural transformation system has yet to be developed. Electrotransformation, a technique by which bacteria are transformed by electroporation,
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Electroporation of ,,s an acute febrile lymphadenitis that can progress into a life-threatening pneumonic illness (.). The disease is usually acquired through the bite of an arthropod vector or from handling contagious wild rabbits. In Europe and Asia, tularemia is often acquired from consumption of contaminated water o
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