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Titlebook: Electrophoretic Separation of Proteins; Methods and Protocol Biji T. Kurien,R. Hal Scofield Book 2019 Springer Science+Business Media, LLC,

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Introduction to Protein Electrophoresis,This chapter briefly discusses the developments in electrophoresis of proteins from Tiselius’ moving-boundary electrophoresis to the modern-day two-dimensional polyacrylamide gel electrophoresis. It also touches upon the staining methods used to visualize total proteins post electrophoresis.
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Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis,etect, identify, and analyze these posttranslational modifications. This new cost-effective methodology will aid the detection of unwanted glycation products in processed foods and may lead to new diagnostics and therapeutics for age-related chronic diseases.
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Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Esolubilization of heat shock proteins (HSP) B1 and B5 and the cytoplasmic adapter protein integrin-linked kinase (ILK) from smooth muscle. Overall, the results demonstrate the importance of optimizing lysis buffers for specific protein solubilization prior to finalizing the experimental workflow.
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Isoelectric Focusing on Non-Denaturing Flatbed Gels,ted over several years were used for analysis using flatbed IEF. Following electrofocusing, the gel is analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane to determine oligoclonality of the anti-Ro 60 containing sera.
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