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Titlebook: Electron Tomography; Three-Dimensional Im Joachim Frank Book 19921st edition Springer Science+Business Media New York 1992 biological.chrom

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Overview: This unique resource details the theory, working methods, andapplications of electron tomographic techniques for imagingasymmetric, noncrystalline biological specimens.978-1-4757-2163-8
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Lisa Hill,Max Douglass,Ravi BaltutisHighest precision in the alignment of projections is one of the prerequisites of tomography. The effects of random translational alignment errors on the reconstruction can be appreciated by considering the much simpler situation where a given number of images containing the same motif are averaged.
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Sample Shrinkage and Radiation Damageconsidered for biological materials of all types in different preparations. The methods employed to minimize the effects of electron irradiation include low-dose imaging techniques and cooling the sample to liquid nitrogen or liquid helium temperatures.
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Safety, Risks and Contraindications, methods will also determine the format in which the reconstruction can be viewed and published. For these reasons methods for the display and visualization of reconstructions are of great interest to investigators who use EM tomography as a tool for their research.
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